Human Rotaviruses

Linda Saif Lab Publications --Human Rotavirus

Azevedo MSP, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV and Saif LJ. 2006. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus . J. Virol. 80:372-382.

Yuan, L., Azevedo, M., Gonzalez, A., Jeong, K., Nguyen, T., Iosef, C., Lewis, P., Herrmann, J., and Saif, L. J. 2005. Mucosal and systemic antibody responses and protection induced by a prime/boost rotavirus-DNA vaccine in a gnotobiotic pig model. Vaccine. 23:3925-3936.

Nguyen, T.V., L. Yuan, M.S.P. Azevedo, K. Jeong, A. Gonzalez and L.J. Saif. 2005. Cytokines transferred from mother to neonates in swine: implications for immunomodulatin of neonatal immunity by maternal cytokines. (submitted).

Nguyen, T.V., L. Yuan, M.S.P. Azevedo, K. Jeong, A. Gonzalez, C. Iosef, K. Lovgren-Bengtsson, B. Morein, P. Lewis and L.J. Saif. 2005. High titer serum maternal antibodies impact protective immunity and B cell responses induced by attenuated rotavirus priming and a rotavirus-like particle-ISCOM boosting vaccine regimen. Vaccine (submitted).

Nguyen, T.V., L. Yuan, M.S.P. Azevedo, K. Jeong, A. Gonzalez, C. Iosef, K. Lovgren-Bengtsson, B. Morein, P. Lewis, and L.J. Saif. 2005. Low titer maternal antibodies both enhance and suppress B cell responses to a combined live attenuated human rotavirus and immunostimulating complex-based rotavirus-like particle vaccine. Vaccine (In preparation).

Azevedo MSP, Yuan L, Jeong K, Gonzalez A, Nguyen T, Pouly S, Gochnauer M, Zhang W, Azevedo A, Saif LJ. 2005. Viremia and nasal and rectal shedding of rotavirus in gnotobiotic pigs inoculated with Wa human rotavirus. J. Virol. 79:5428-5436.

Yuan, L., A. Geyer, D.C. Hodgins, V. Parreno, Z. Fan, L.A. Ward, M.S.P. Azevedo, C. Iosef and L.J. Saif. 2004. Effects of low titre circulating maternal antibodies on protective immunity and development of active antibody responses in gnotobiotic pigs inoculated with an attenuated human rotavirus. Clin. Diag. Lab Immunol. (In preparation).

Gonzalez, A.M. T.V. Nguyen, M.S.P. Azevedo, K. Jeong, F. Agarib, C. Iosef, K. Chang, K. Lovgren-Bengtsson, B. Morein, and L.J. Saif. 2004. Antibody responses to human rotavirus (HRV) in gnotobiotic pigs following a new prime-boost vaccine strategy using oral attenuated HRV priming and intranasal VP2/6 rotavirus-like particle (VLP) boosting with ISCOM. Clin. Exp. Immunol.135:361-372.

Azevedo, M.S.P., L. Yuan, C. Iosef, K-O. Chang, T.V. Nguyen, Y. Kim, and L. J. Saif. 2004. Magnitude of serum and intestinal antibody responses induced by sequential replicating and non-replicating rotavirus vaccines in gnotobiotic pigs and correlation with protection. Clin Diag Lab Immunol 11:12-20.

Nguyen TV, Iosef C, Jeong K, Kim Y, Chang KO, Lovgren-Bengtsson K, Morein B, Azevedo MS, Lewis P, Nielsen P, Yuan L, Saif LJ. 2003. Protection and antibody responses to oral priming by attenuated human rotavirus followed by oral boosting with 2/6-rotavirus-like particles with immunostimulating complexes in gnotobiotic pigs. Vaccine 21:4059-70.

Yuan, L. and L.J. Saif. 2002. Induction of mucosal immune responses and protection against enteric viruses: rotavirus infection of gnotobiotic pigs as a model. Vet Immunol Immunopathol 87:147-60.

Iosef, C., T. Van Nguyen, K. Jeong, K. Bengtsson, B. Morein, Y. Kim, K.O. Chang, M.S. Azevedo, L. Yuan, P. Nielsen, and L.J. Saif. 2002. Systemic and intestinal antibody secreting cell responses and protection in gnotobiotic pigs immunized orally with attenuated Wa human rotavirus and Wa 2/6-rotavirus-like-particles associated with immunostimulating complexes. Vaccine 20 :1741-53.

Iosef, C., K.O. Chang, M.S. Azevedo, and L.J. Saif. 2002. Systemic and intestinal antibody responses to NSP4 enterotoxin of Wa human rotavirus in a gnotobiotic pig model of human rotavirus disease. J Med Virol 68:119-28.

Yuan, L., C. Iosef, M.S. Azevedo, Y. Kim, Y. Qian, A. Geyer, T.V. Nguyen, K.O. Chang, and L.J. Saif. 2001. Protective immunity and antibody-secreting cell responses elicited by combined oral attenuated Wa human rotavirus and intranasal Wa 2/6-VLPs with mutant Escherichia coli heat-labile toxin in gnotobiotic pigs. J Virol 75:9229-38.

Yuan, L., A. Geyer, and L.J. Saif. 2001. Short-term immunoglobulin A B-cell memory resides in intestinal lymphoid tissues but not in bone marrow of gnotobiotic pigs inoculated with Wa human rotavirus. Immunology 103:188-98.

Yang, K., S. Wang, K.O. Chang, S. Lu, L.J. Saif, H.B. Greenberg, J.P. Brinker, and J.E. Herrmann. 2001. Immune responses and protection obtained with rotavirus VP6 DNA vaccines given by intramuscular injection. Vaccine 19:3285-91.

Chang, K.O., O.H. Vandal, L. Yuan, D.C. Hodgins, and L.J. Saif. 2001. Antibody-secreting cell responses to rotavirus proteins in gnotobiotic pigs inoculated with attenuated or virulent human rotavirus. J Clin Microbiol 39:2807-13.

Yuan, L., A. Geyer, D.C. Hodgins, Z. Fan, Y. Qian, K.O. Chang, S.E. Crawford, V. Parreno, L.A. Ward, M.K. Estes, M.E. Conner, and L.J. Saif. 2000. Intranasal administration of 2/6-rotavirus-like particles with mutant Escherichia coli heat-labile toxin (LT-R192G) induces antibody- secreting cell responses but not protective immunity in gnotobiotic pigs. J Virol 74:8843-53.

Hodgins, D.C., S.Y. Kang, L. deArriba, V. Parreno, L.A. Ward, L. Yuan, T. To, and L.J. Saif. 1999. Effects of maternal antibodies on protection and development of antibody responses to human rotavirus in gnotobiotic pigs. J Virol 73:186-97.

Parreno, V., D.C. Hodgins, L. de Arriba, S.Y. Kang, L. Yuan, L.A. Ward, T.L. To, and L.J. Saif. 1999. Serum and intestinal isotype antibody responses to Wa human rotavirus in gnotobiotic pigs are modulated by maternal antibodies. J Gen Virol 80 ( Pt 6):1417-28.

Yuan, L., S.Y. Kang, L.A. Ward, T.L. To, and L.J. Saif. 1998. Antibody-secreting cell responses and protective immunity assessed in gnotobiotic pigs inoculated orally or intramuscularly with inactivated human rotavirus. J Virol 72:330-8.

To, T.L., L.A. Ward, L. Yuan, and L.J. Saif. 1998. Serum and intestinal isotype antibody responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of disease. J Gen Virol 79 ( Pt 11):2661-72.

Saif, L., L. Yuan, L. Ward, and T. To. 1997. Comparative studies of the pathogenesis, antibody immune responses, and homologous protection to porcine and human rotaviruses in gnotobiotic piglets. Adv Exp Med Biol 412:397-403.

Yuan, L., L.A. Ward, B.I. Rosen, T.L. To, and L.J. Saif. 1996. Systematic and intestinal antibody-secreting cell responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of disease. J Virol 70:3075-83.

Ward, L.A., L. Yuan, B.I. Rosen, T.L. To, and L.J. Saif. 1996. Development of mucosal and systemic lymphoproliferative responses and protective immunity to human group A rotaviruses in a gnotobiotic pig model. Clin Diagn Lab Immunol 3:342-50.

Ward, L.A., B.I. Rosen, L. Yuan, and L.J. Saif. 1996. Pathogenesis of an attenuated and a virulent strain of group A human rotavirus in neonatal gnotobiotic pigs. J Gen Virol 77 ( Pt 7):1431-41.

Tsunemitsu, H., B. Jiang, and L.J. Saif. 1996. Sequence comparison of the VP7 gene encoding the outer capsid glycoprotein among animal and human group C rotaviruses. Arch Virol 141:705-13.

Saif, L.J., L.A. Ward, L. Yuan, B.I. Rosen, and T.L. To. 1996. The gnotobiotic piglet as a model for studies of disease pathogenesis and immunity to human rotaviruses. Arch Virol Suppl 12:153-61.

Jiang, B., H. Tsunemitsu, P.H. Dennehy, I. Oishi, D. Brown, R.D. Schnagl, M. Oseto, Z.Y. Fang, L.F. Avendano, L.J. Saif, and R.I. Glass. 1996. Sequence conservation and expression of the gene encoding the outer capsid glycoprotein among human group C rotaviruses of global distribution. Arch Virol 141:381-90.

1996 Conner, M.E., S.E. Crawford, C. Barone, C. O'Neal, Y.J. Zhou, F. Fernandez, A. Parwani, L.J. Saif, J. Cohen, and M.K. Estes. 1996. Rotavirus subunit vaccines. Arch Virol Suppl 12:199-206.

Saif, L.J. and B. Jiang. 1994. Nongroup A rotaviruses of humans and animals. Curr Top Microbiol Immunol 185:339-71.

Gouvea, V., C. Ramirez, B. Li, N. Santos, L. Saif, H.F. Clark, and Y. Hoshino. 1993. Restriction endonuclease analysis of the vp7 genes of human and animal rotaviruses. J Clin Microbiol 31:917-23.

Schaller, J.P., L.J. Saif, C.T. Cordle, E. Candler Jr, T.R. Winship, and K.L. Smith. 1992. Prevention of human rotavirus-induced diarrhea in gnotobiotic piglets using bovine antibody. J Infect Dis 165:623-30.

Qian, Y.A., B.M. Jiang, L.J. Saif, S.Y. Kang, Y. Ishimaru, Y. Yamashita, M. Oseto, and K.Y. Green. 1991. Sequence conservation of gene 8 between human and porcine group C rotaviruses and its relationship to the VP7 gene of group A rotaviruses. Virology 182:562-9.

Qian, Y., L.J. Saif, A.Z. Kapikian, S.Y. Kang, B. Jiang, Y. Ishimaru, Y. Yamashita, M. Oseto, and K.Y. Green. 1991. Comparison of human and porcine group C rotaviruses by northern blot hybridization analysis. Arch Virol 118:269-77.

Gouvea, V., J.R. Allen, R.I. Glass, Z.Y. Fang, M. Bremont, J. Cohen, M.A. McCrae, L.J. Saif, P. Sinarachatanant, and E.O. Caul. 1991. Detection of group B and C rotaviruses by polymerase chain reaction. J Clin Microbiol 29:519-23.

Penaranda, M.E., W.D. Cubitt, P. Sinarachatanant, D.N. Taylor, S. Likanonsakul, L. Saif, and R.I. Glass. 1989. Group C rotavirus infections in patients with diarrhea in Thailand, Nepal, and England. J Infect Dis 160:392-7.

Fang, Z.Y., R.I. Glass, M. Penaranda, H. Dong, S.S. Monroe, L. Wen, M.K. Estes, J. Eiden, R.H. Yolken, L. Saif, and a.l. et. 1989. Purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins. J Virol 63:2191-7.

Saif, L. J., B. I. Rosen, S.‑Y. Kang, and K. L. Miller. 1988. Cell culture propagation of rotavirus. J. Tissue Culture Meth. 11:147‑156.

Nakata, S., M.K. Estes, D.Y. Graham, R. Loosle, H. Tao, S.H. Wang, L.J. Saif, and J.L. Melnick. 1986. Antigenic characterization and ELISA detection of adult diarrhea rotaviruses. J Infect Dis 154:448-55.

Saif, L. J. and K. W. Theil. 1985. Antigenically distinct rotaviruses of human and animal origin. Infect. Diarrhea in the Young. S. Tzipori, Ed, Elseiver, The Netherlands, pp. 208‑214.

Riepenhoff-Talty, M., L.J. Saif, H.J. Barrett, H. Suzuki, and P.L. Ogra. 1983. Potential spectrum of etiological agents of viral enteritis in hospitalized infants. J Clin Microbiol 17:352-6.

Wyatt, R.G., W.D. James, E.H. Bohl, K.W. Theil, L.J. Saif, A.R. Kalica, H.B. Greenberg, A.Z. Kapikian, and R.M. Chanock. 1980. Human rotavirus type 2: cultivation in vitro. Science 207:189-91.