Porcine Rotaviruses

Linda Saif Lab Publications-Porcine Rotaviruses

Yuan, L. Stevenson, G., Saif, L. J. 2005. Rotavirus and Reovirus. In: Diseases of Swine. 9th Edition. Edited by Zimmerman, J. J., et al., Iowa State University Press, Ames, Iowa (in press).

Jiang, B., L.J. Saif, J.R. Gentsch, and R.I. Glass. 2000. Completion of the four large gene sequences of porcine group C Cowden rotavirus. Virus Genes 20:193-4.

Saif, L.J. 1999. Enteric viral infections of pigs and strategies for induction of mucosal immunity. Adv Vet Med 41:429-46.

Saif, L.J. 1999. Comparative pathogenesis of enteric viral infections of swine. Adv Exp Med Biol 473:47-59.

Kim, Y., K.O. Chang, B. Straw, and L.J. Saif. 1999. Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs. J Clin Microbiol 37:1484-8.

Chang, K.O., Y.J. Kim, and L.J. Saif. 1999. Comparisons of nucleotide and deduced amino acid sequences of NSP4 genes of virulent and attenuated pairs of group A and C rotaviruses. Virus Genes 18:229-33.

Zhang, M. C.Q. Zeng, Y. Dong, J.M. Ball, L.J. Saif, A.P. Morris, and M.K. Estes. 1998. Mutations in rotavirus nonstructural glycoprotein NSP4 are associated with altered virus virulence. J Virol 72:3666-72.

Saif, L.J. and M.B. Wheeler. 1998. WAPping gastroenteritis with transgenic antibodies. Nat Biotechnol 16:334-5.

Saif, L.J. and F.M. Fernandez. 1996. Group A rotavirus veterinary vaccines. J Infect Dis 174 Suppl 1:S98-106.

Hoshino, Y., L.J. Saif, S.Y. Kang, M.M. Sereno, W.K. Chen, and A.Z. Kapikian. 1995. Identification of group A rotavirus genes associated with virulence of a porcine rotavirus and host range restriction of a human rotavirus in the gnotobiotic piglet model. Virology 209:274-80.

Chen, W.K., T. Campbell, J. VanCott, and L.J. Saif. 1995. Enumeration of isotype-specific antibody-secreting cells derived from gnotobiotic piglets inoculated with porcine rotaviruses. Vet Immunol Immunopathol 45:265-84.

Rosen, B.I., A.V. Parwani, S. Lopez, J. Flores, and L.J. Saif. 1994. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol 32:311-7.

Ojeh, C.K., A.V. Parwani, B.M. Jiang, K.W. Theil, B.I. Rosen, and L.J. Saif. 1993. Characterization of field isolates of porcine group C rotaviruses using gene 5 (VP6) and gene 8 (VP7) cDNA probes. J Vet Diagn Invest 5:434-8.

Kang, S.Y., D.A. Benfield, M. Gorziglia, and L.J. Saif. 1993. Characterization of the neutralizing epitopes of VP7 of the Gottfried strain of porcine rotavirus. J Clin Microbiol 31:2291-7.

Tsunemitsu, H., C.K. Ojeh, B. Jiang, R.A. Simkins, P.A. Weilnau, and L.J. Saif. 1992. Production and characterization of monoclonal antibodies to porcine group C rotaviruses cross-reactive with group A rotaviruses. Virology 191:272-81.

Tsunemitsu, H., B. Jiang, Y. Yamashita, M. Oseto, H. Ushijima, and L.J. Saif. 1992. Evidence of serologic diversity within group C rotaviruses. J Clin Microbiol 30:3009-12.

Tsunemitsu, H., B. Jiang, and L.J. Saif. 1992. Detection of group C rotavirus antigens and antibodies in animals and humans by enzyme-linked immunosorbent assays. J Clin Microbiol 30:2129-34.

Rosen, B.I., A.V. Parwani, M. Gorziglia, G. Larralde, and L.J. Saif. 1992. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. J Clin Microbiol 30:2644-52.

Ojeh, C.K., H. Tsunemitsu, R.A. Simkins, and L.J. Saif. 1992. Development of a biotin-streptavidin-enhanced enzyme-linked immunosorbent assay which uses monoclonal antibodies for detection of group C rotaviruses. J Clin Microbiol 30:1667-73.

Jiang, B.M., H. Tsunemitsu, Y. Qian, K.Y. Green, M. Oseto, Y. Yamashita, and L.J. Saif. 1992. Analysis of the genetic diversity of genes 5 and 6 among group C rotaviruses using cDNA probes. Arch Virol 126:45-56.

Jiang, B.M. and L.J. Saif. 1992. In vitro transcription and translation of genomic RNA from a porcine group C rotavirus. Arch Virol 124:181-5.

Qian, Y.A., B.M. Jiang, L.J. Saif, S.Y. Kang, C.K. Ojeh, and K.Y. Green. 1991. Molecular analysis of the gene 6 from a porcine group C rotavirus that encodes the NS34 equivalent of group A rotaviruses. Virology 184:752-7.

Ojeh, C.K., B.M. Jiang, H. Tsunemitsu, S.Y. Kang, P.A. Weilnau, and L.J. Saif. 1991. Reactivity of monoclonal antibodies to the 41-kilodalton protein of porcine group C rotavirus with homologous and heterologous rotavirus serogroups in immunofluorescence tests. J Clin Microbiol 29:2051-5.

Kang, S.Y. and L.J. Saif. 1991. Production and characterization of monoclonal antibodies against an avian group A rotavirus. Avian Dis 35:563-71.

Jiang, B.M., Y. Qian, H. Tsunemitsu, K.Y. Green, and L.J. Saif. 1991. Analysis of the gene encoding the outer capsid glycoprotein (VP7) of group C rotaviruses by northern and dot blot hybridization. Virology 184:433-6.

Cordle, C.T., J.P. Schaller, T.R. Winship, E.L. Candler, M.D. Hilty, K.L. Smith, L.J. Saif, E.M. Kohler, and S. Krakowka. 1991. Passive immune protection from diarrhea caused by rotavirus or E. coli: an animal model to demonstrate and quantitate efficacy. Adv Exp Med Biol 310:317-27.

Rosen, B.I., L.J. Saif, D.J. Jackwood, and M. Gorziglia. 1990. Hybridization probes for the detection and differentiation of two serotypes of porcine rotavirus. Vet Microbiol 24:327-39.

Rosen, B.I., L.J. Saif, D.J. Jackwood, and M. Gorziglia. 1990. Serotypic differentiation of group A rotaviruses with porcine rotavirus gene 9 probes. J Clin Microbiol 28:2526-33.

Kassuba, A., L.J. Saif, and H.B. Greenberg. 1990. Subgroup classification of porcine group-A rotaviruses, using monoclonal antibodies in an enzyme-linked immunosorbent assay. Am J Vet Res 51:938-44.

Jiang, B.M., L.J. Saif, S.Y. Kang, and J.H. Kim. 1990. Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus. J Virol 64:3171-8.

Kang, S.Y., L.J. Saif, and K.L. Miller. 1989. Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotypes of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol 27:2744-50.

Saif, L.J., L.A. Terrett, K.L. Miller, and R.F. Cross. 1988. Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. J Clin Microbiol 26:1277-82.

Hoshino, Y., L.J. Saif, M.M. Sereno, R.M. Chanock, and A.Z. Kapikian. 1988. Infection immunity of piglets to either VP3 or VP7 outer capsid protein confers resistance to challenge with a virulent rotavirus bearing the corresponding antigen. J Virol 62:744-8.

Terrett, L.A., L.J. Saif, K.W. Theil, and E.M. Kohler. 1987. Physicochemical characterization of porcine pararotavirus and detection of virus and viral antibodies using cell culture immunofluorescence. J Clin Microbiol 25:268-72.

Terrett, L.A. and L.J. Saif. 1987. Serial propagation of porcine group C rotavirus (pararotavirus) in primary porcine kidney cell cultures. J Clin Microbiol 25:1316-9.

Hoblet, K.H., L.J. Saif, E.M. Kohler, K.W. Theil, S. Bech-Nielsen, and G.A. Stitzlein. 1986. Efficacy of an orally administered modified-live porcine-origin rotavirus vaccine against postweaning diarrhea in pigs. Am J Vet Res 47:1697-703.

Hoblet, K.H., E.M. Kohler, L.J. Saif, K.W. Theil, and W.L. Ingalls. 1986. Study of porcine postweaning diarrhea involving K88(-) hemolytic Escherichia coli. Am J Vet Res 47:1910-2.

Theil, K.W., L.J. Saif, P.D. Moorhead, and R.E. Whitmoyer. 1985. Porcine rotavirus-like virus (group B rotavirus): characterization and pathogenicity for gnotobiotic pigs. J Clin Microbiol 21:340-5.

Theil, K.W. and L.J. Saif. 1985. In vitro detection of porcine rotavirus-like virus (group B rotavirus) and its antibody. J Clin Microbiol 21:844-6.

Saif, L. J. 1985. Typical and atypical rotaviruses of swine. Proceedings George A. Young Conference. Lincoln, Nb, p. 73‑81.

Saif, L. J. 1985. Passive immunity to coronavirus and rotavirus infections in swine and cattle: enhancement by maternal vaccination. Infect. Diarrhea in the Young. S. Tzipori, Ed, Elseiver, The Netherlands, pp. 456‑467.

Bohl, E.H., K.W. Theil, and L.J. Saif. 1984. Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol 19:105-11.

Mahan, D.C. and L.J. Saif. 1983. Efficacy of vitamin C supplementation for weanling swine. J Anim Sci 56:631-9.

Bohl, E.H., L.J. Saif, K.W. Theil, A.G. Agnes, and R.F. Cross. 1982. Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs. J Clin Microbiol 15:312-9.

Theil, K.W., C.M. McCloskey, L.J. Saif, D.R. Redman, E.H. Bohl, D.D. Hancock, E.M. Kohler, and P.D. Moorhead. 1981. Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis. J Clin Microbiol 14:273-80.

Saif, L.J., E.H. Bohl, K.W. Theil, R.F. Cross, and J.A. House. 1980. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol 12:105-11.

Theil, K.W., L.J. Saif, E.H. Bohl, A.G. Agnes, and E.M. Kohler. 1979. Concurrent porcine rotaviral and transmissible gastroenteritis viral infections in a three-day-old conventional pig. Am J Vet Res 40:719-21.

Theil, K.W., E.H. Bohl, and L.J. Saif. 1978. Techniques for rotaviral propagation. J Am Vet Med Assoc 173:548-51.

Saif, L.J., K.W. Theil, and E.H. Bohl. 1978. Morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells. J Gen Virol 39:205-17.

Bohl, E.H., E.M. Kohler, L.J. Saif, R.F. Cross, A.G. Agnes, and K.W. Theil. 1978. Rotavirus as a cause of diarrhea in pigs. J Am Vet Med Assoc 172:458-63.